ACTH Stimulation Test in Dogs: What This Blood Test Detects
What is an ACTH stimulation test?
The ACTH stimulation test is a specialized blood test used to evaluate how well your dog’s adrenal glands are functioning. It helps diagnose and monitor hormonal disorders that affect cortisol production.
What does the ACTH stim test check for?
This test is most commonly used to diagnose:
- Addison's disease (low cortisol production)
- Cushing's disease (excess cortisol production)
It can also be used to monitor dogs already receiving treatment for these conditions.
How does the ACTH stimulation test work?
The test measures your dog’s cortisol levels before and after receiving an injection of synthetic ACTH (a hormone that stimulates the adrenal glands).
Step-by-step process:
- A baseline blood sample is taken
- An ACTH injection is given
- A second blood sample is taken about 1 hour later
The results show how the adrenal glands respond to stimulation.
Why would my dog need an ACTH stim test?
Your veterinarian may recommend this test if your dog shows symptoms such as:
Possible signs of Addison’s disease:
- Weakness or collapse
- Vomiting or diarrhea
- Weight loss
- Low energy
Possible signs of Cushing’s disease:
- Increased thirst and urination
- Increased appetite
- Panting
- Hair loss or thinning coat
These symptoms can overlap with other conditions, making diagnostic testing essential.
How long does the ACTH stimulation test take?
The test typically takes about 1 to 2 hours from start to finish. Your pet will stay at the hospital during this time.
Is the ACTH stim test safe?
Yes, the test is considered very safe. Side effects from the ACTH injection are rare.
How accurate is the ACTH stimulation test?
The ACTH stim test is:
- The gold standard for diagnosing Addison’s disease
- Commonly used (along with other tests) to diagnose Cushing’s disease
- Important for monitoring treatment effectiveness
What happens after the test?
Once results are available, your veterinarian will:
- Review cortisol levels
- Confirm or rule out adrenal disease
- Recommend treatment or additional testing if needed
ACTH Stimulation Test FAQ
Does my dog need to fast before the test?
Fasting may be recommended, depending on your pet’s situation. We’ll provide specific instructions before your visit.
Is this test painful for my dog?
Aside from a small needle for blood draws and the injection, the procedure is minimally invasive and well tolerated.
How soon will I get results?
Results are often available within 24 hours, depending on the lab.
Schedule an ACTH Stimulation Test in Cincinnati
If your dog is showing signs of a hormonal imbalance, early testing can make a critical difference.
At Deer Park Vet Hospital, we provide accurate diagnostic testing and personalized care to help your pet feel their best.
Call today or request an appointment online.